Tessitura explores how contemporary opera singers navigate the traditional vocal categories of their art form, interweaving their personal stories with historical context. Central to this exploration is musicologist Dr. Naomi André, who provides context about the legacy of castrati and trouser roles – grounding the film in opera's long association with dismantling gender binaries. But at the heart of the film are three singers on unique journeys: Breanna Sinclairé, a soprano dreaming of performing traditional leading roles; Lucas Bouk, a baritone searching for his place in modern opera repertoire; and Katherine Goforth, a rising star who is committed to redefining the opera’s boundaries.